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超高速視頻級原子力顯微鏡HS-AFM

價    格:3800000

產    地:日本更新時間:2022/11/15 14:31:39

品    牌:RIBM型    號:HS-AFM

狀    態:正常點擊量:70

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北京佰司特貿易有限責任公司

聯 系 人:周亮

電     話:010-67751532

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超高速視頻級原子力顯微鏡HS-AFM(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大學 Prof. Ando 教授團隊研發,日本RIBM公司商業化的產品,可以達到視頻級成像的商業化原子力顯微鏡。HS-AFM突破了傳統原子力顯微鏡“掃描成像速慢”的限制,能夠在液體環境下超快速動態成像,分辨率為納米水平。


超高速視頻級原子力顯微鏡HS-AFM(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大學 Prof. Ando 教授團隊研發,日本RIBM公司(生體分子計測研究所株式會社,Research Institute of Biomolecule Metrology Co., Ltd)商業化的產品,可以達到視頻級成像的商業化原子力顯微鏡。HS-AFM突破了傳統原子力顯微鏡“掃描成像速慢”的限制,能夠在液體環境下Chao快速動態成像,分辨率為納米水平。樣品Wu需特殊固定,不影響生物分子的活性,尤其適用于生物大分子互作動態觀測。超高速視頻級原子力顯微鏡HS-AFM主要有兩種型號,SS-NEX樣品掃描(Sample-Scanning HS-AFM)以及PS-NEX探針掃描(Probe-Scanning HS-AFM)。推出至今,全有100多位用戶,發表 SCI 文章 300 余篇,包括Science, Nature, Cell 等雜志。

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相較于市場上的原子力顯微鏡成像設備,HS-AFM突破了 “掃描成像速慢”的限制,掃描速度高可達 20 frame/s,并且有 4 種掃描臺可供選擇。樣品Wu需特殊固定染色,不影響生物分子的活性,尤其適用于生物大分子互作動態觀測。液體環境下直接檢測,超快速動態成像,分辨率為納米水平。探針小,適用于生物樣品;懸臂探針共振頻率高,彈簧系數小,避免了對生物樣品等的損傷。懸臂探針可自動漂移校準,適用于長時間觀測。采用動態PID控制,高速掃描時仍可獲得清晰的圖像。XY軸分辨率2nm;Z軸分辨率0.5nm。

HS-AFM 擁有超高掃描速率與原子級別分辨率,而且具有操作的簡易性,使得對單分子動態過程的捕捉變得十分方便,為科研工作者研所和理解生物物理、生物化學、分子生物學、病毒學以及生物醫學等領域的單分子動態過程提供了一款強大的工具。

新的HS-AFM采用了新的高頻微懸臂架構,Geng低噪音、Geng高穩定性的2控制器,高速掃描器,緩沖防震設計,主動阻尼,動態PID,驅動算法You 化,多種Qian沿技術,可以實現在超高速下獲取高分辨的生物樣品信息。新系統整合了基于工作流程的操作軟件,直觀的用戶界面與流程化、自動化的設置使得研究人員可以專注于實驗設計,不需要復雜的操作和條件設置,快速獲取數據,加速研究的產出。




產品參數

超高速視頻級原子力顯微鏡HS-AFM(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大學 Prof. Ando 教授團隊研發,日本RIBM公司商業化的產品,可以達到視頻級成像的商業化原子力顯微鏡。HS-AFM突破了傳統原子力顯微鏡“掃描成像速慢”的限制,能夠在液體環境下超快速動態成像,分辨率為納米水平。


★? ?高頻微懸臂

彈性系數: ? 0.1 N/m ??

曲率半徑: ?<10 nm 

共振頻率: ? 400-600kHz in liquid

★? ?高速掃描臺

20 frames/s. ? (Standard scanner)

★? ?緩沖防震+主動阻尼+動態PID+算法You 化

緩沖防震

主動阻尼

動態PID控制:可自動改變反饋增益,HS-AFM在高速掃描條件下仍可獲得清晰的圖像

探針自動漂移校準,適用于長時間樣品觀測




產品介紹

超高速視頻級原子力顯微鏡HS-AFM(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大學 Prof. Ando 教授團隊研發,日本RIBM公司商業化的產品,可以達到視頻級成像的商業化原子力顯微鏡。HS-AFM突破了傳統原子力顯微鏡“掃描成像速慢”的限制,能夠在液體環境下超快速動態成像,分辨率為納米水平。


原子力顯微鏡(Atomic Force Microscope,AFM),一種可用來研究包括絕緣體在內的固體材料表面結構的分析儀器。它通過檢測待測樣品表面和一個微型力敏感元件之間的極微弱的原子間相互作用力來研究物質的表面結構及性質。將一對微弱力極端敏感的微懸臂一端固定,另一端的微小針尖接近樣品,這時它將與其相互作用,作用力將使得微懸臂發生形變或運動狀態發生變化。掃描樣品時,利用傳感器檢測這些變化,就可獲得作用力分布信息,從而以納米級分辨率獲得表面形貌結構信息及表面粗糙度信息。原子力顯微鏡可以測量材料物理性質、力學性能、磁學性能、熱學性能、電學性能等方面的一些特征信息,但在掃描成像速度上一直存在局限性,太慢的掃描速度導致原子力顯微鏡無法捕捉到分子間的相互作用過程和一些快速的分子動態變化。

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日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的的應用領域:

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從單分子到單細胞,都可直接觀測

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1、肌動蛋白

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2、CRISPR-Cas9

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3、膜聯蛋白

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4、IgG

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5、活細胞

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6、細菌視紫紅質

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7、DNA納米結構

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8、仿生聚合物

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日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的視頻案例

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1:IgG

在溶液中觀察到抗體(IgG)。

IgG呈"Y"形,兩個Fab區區分清晰。

由于錨定能力較弱,IgG保持其抗原結合能力。

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2:Plasmid DNA

傳統AFM在沒有強錨定的情況下,DNA分子圖像出現擺動。

然而,強錨定可能會削弱真實的結構和行為。

HS-AFM能清晰顯示質粒的結構和運動,無強錨定。

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3:DNA內切酶的消化:DNase I

DNA酶I是一種隨機消化DNA的核酸內切酶。視頻中的箭頭表示DNase I消化DNA的部分。

請參考從DNA末端消化的核酸外切酶Bal31的視頻。

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4:DNA外切酶消化:Bal31

Bal31是一種從DNA鏈末端消化DNA的核酸外切酶。

視頻顯示Bal31的活性沿著DNA移動,并逐漸從DNA鏈的末端消化。

DNA分子被完全消化,但環狀DNA未被消化。高光點是Bal31分子,它們與DNA的不同位置結合。
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5:DNA聚合酶的DNA延伸:Phi29

雙鏈DNA(黃色)隨著時間的推移而拉長。單鏈λDNA作為模具固定在基板上。

由于從隨機六聚體引物(Red)結合到λDNA模體,phi29聚合酶(Black)以dNTP為底物合成互補DNA。
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6:鏈親和素2D晶體中的點缺陷

成功地觀察到點缺陷在晶體中的擴散。

從圖像上看,兩個單空位缺陷的軌跡跟蹤相對于晶格的兩個軸是明顯的各向異性的。

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日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的文獻列表

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TitleJournal
Biophysical reviews top five: atomic force microscopy in biophysicsBiophysical Reviews
Reconstruction of Three-Dimensional Conformations of Bacterial
ClpB from High-Speed Atomic-Force-Microscopy Images
Frontiers in Molecular
Biosciences
A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes
Chemical Science
DNA Nanotechnology to Disclose Molecular Events at the Nanoscale
and Mesoscale Levels
Springer Nature
Quantitative description of a contractile macromolecular machineScience Advances
Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ
Visualized by High-Speed Atomic Force Microscopy
International Journal of
Molecular Sciences 2021, Vol. 22, Page 1697
Localization atomic force microscopyNature 2021 594:7863
Movements of mycoplasma mobile gliding machinery detected by
high-speed atomic force microscopy
mBio
An ultra-wide scanner for large-area high-speed atomic force
microscopy with megapixel resolution
Scientific Reports 2021 11:1
A molecularly engineered, broad-spectrum anti-coronavirus lectin
inhibits SARS-CoV-2 and MERS-CoV infection in vivo
Research Square
Influenza virus ribonucleoprotein complex formation occurs in the
nucleolus
bioRxiv
Tardigrade Secretory-Abundant Heat-Soluble Protein Has a Flexible
β-Barrel?Structure?in?Solution?and?Keeps?This?Structure?in
Dehydration
Journal of Physical Chemistry B
Ultrastructure of influenza virus ribonucleoprotein complexes during
viral RNA synthesis
Communications Biology on
A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes
Chemical Science
Deformation of microtubules regulates translocation dynamics of
kinesin
Science Advances
Unraveling the host-selective toxic interaction of cassiicolin with lipid
membranes and its cytotoxicity
bioRxiv
Nanostructure and thermoresponsiveness of poly( N -isopropyl
methacrylamide)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization
RSC Advances
JRAB/MICAL-L2?undergoes?liquid–liquid?phase?separation?to?form
tubular recycling endosomes
Communications Biology 2021
4:1
Correlation of membrane protein conformational and functional
dynamics
Nature Communications 2021
12:1
Non-close-packed arrangement of soft elastomer microspheres on
solid substrates
RSC Advances
Folding?RNA–Protein?Complex?into?Designed?NanostructuresMethods in Molecular Biology
A glutamine sensor that directly activates TORC1Communications Biology 2021
4:1
Architecture of zero-latency ultrafast amplitude detector for high-
speed atomic force microscopy
Applied Physics Letters
Correlative AFM and fluorescence imaging demonstrate nanoscale
membrane remodeling and ring-like and tubular structure formation by septins
Nanoscale
Desiccation-induced fibrous condensation of CAHS protein from an
anhydrobiotic tardigrade
bioRxiv
Construction?of?ferritin?hydrogels?utilizing?subunit–subunit
interactions
PLOS ONE
Monomeric?α-synuclein?(αS)?inhibits?amyloidogenesis?of?human
prion?protein?(hPrP)?by?forming?a?stable?αS-hPrP?hetero-dimer.
Prion
Influence of protein adsorption on aggregation in prefilled syringesJournal of Pharmaceutical
Sciences
Dynamic mechanisms of CRISPR interference by Escherichia coli
CRISPR-Cas3
bioRxiv
An RNA Triangle with Six Ribozyme Units Can Promote a Trans-
Splicing Reaction through Trimerization of Unit Ribozyme Dimers
Applied Sciences
Faster high-speed atomic force microscopy for imaging of
biomolecular processes Review of Scientific Instruments ARTICLE scitation.org/journal/rsi Faster high-speed atomic force microscopy for imaging of biomolecular processes
Rev. Sci. Instrum
Title: Identification of lectin receptors for conserved SARS-CoV-2
glycosylation sites
bioRxiv
Structural and dynamics analysis of intrinsically disordered proteins
by high-speed atomic force microscopy
Nature Nanotechnology
Millisecond Conformational Dynamics of Skeletal Myosin II Power
Stroke Studied by High-Speed Atomic Force Microscopy
ACS Nano
High-Speed Atomic Force Microscopy Reveals Spatiotemporal
Dynamics of Histone Protein H2A Involution by DNA Inchworming
The Journal of Physical
Chemistry Letters
Nanostructures, Thermoresponsiveness, and Assembly Mechanism
of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization
Langmuir
Chained structure of dimeric F 1-like ATPase in Mycoplasma mobile
gliding machinery 4
bioRxiv
Lipid Membrane Interaction of Peptide/DNA Complexes Designed for
Gene Delivery
Langmuir
High-Speed Atomic Force Microscopy to Study Myosin MotilityAdvances in Experimental
Medicine and Biology
Single-molecule level dynamic observation of disassembly of the
apo-ferritin cage in solution
Physical Chemistry Chemical
Physics
Atomic Force Microscopy of Biomembranes : A Tool for Studying the
Dynamic Behavior of Membrane Proteins
New Techniques for Studying
Biomembranes
Adenosine leakage from perforin-burst extracellular vesicles inhibits
perforin secretion by cytotoxic T-lymphocytes
PLOS ONE
High-Speed Atomic Force Microscopy Reveals the Structural
Dynamics?of?the?Amyloid-β?and?Amylin?Aggregation?Pathways
International Journal of
Molecular Sciences 2020, Vol. 21, Page 4287
Molecular mechanism of the recognition of bacterially cleaved
immunoglobulin by the immune regulatory receptor LILRA2
Journal of Biological Chemistry
Biological physics by high-speed atomic force microscopyPhilosophical Transactions of
the Royal Society A: Mathematical, Physical and Engineering Sciences
Viral RNA recognition by LGP2 and MDA5, and activation of signaling
through step-by-step conformational changes
Nucleic Acids Research
Key Nucleation Stages and Associated Molecular Determinants and
Processes in pH-Induced Formation of Amyloid Beta Oligomers as Revealed by High-Speed AFM
bioRxiv
Novel Babesia bovis _ exported proteins that modify properties of
infected red blood cells
PLoS Pathogens
DNA origami demonstrate the unique stimulatory power of single
pMHCs as T-cell antigens
Proceedings of the National
Academy of Sciences
Structural insights into the mechanism of rhodopsin
phosphodiesterase
Nature Communications
Structural insights into the mechanism of rhodopsin
phosphodiesterase
Nature Communications
High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes
Nano Letters
DNA Ring Motif with Flexible JointsMicromachines
Nanopores: a versatile tool to study protein dynamicsEssays in Biochemistry
Geometrical Characterization of Glass Nanopipettes with Sub-10 nm
Pore Diameter by Transmission Electron Microscopy
Analytical Chemistry
Carbon nanotube porin diffusion in mixed composition supported
lipid bilayers
Scientific Reports
One-Step Calibration of AFM in LiquidFrontiers in Physics
Nanoscale interaction of RecG with mobile fork DNANanoscale Advances
Convergent evolution of processivity in bacterial and fungal
cellulases
Proceedings of the National
Academy of Sciences
Convergent evolution of processivity in bacterial and fungal
cellulases
Proceedings of the National
Academy of Sciences of the United States of America
Interaction of the motor protein SecA and the bacterial protein
translocation channel SecYEG in the absence of ATP
Nanoscale Advances
Nanoreporter of an Enzymatic Suicide Inactivation PathwayNano Letters
High-speed atomic force microscopy highlights new molecular
mechanism of daptomycin action
Nature Communications
Direct visualization of the conformational change of FUS/TLS upon
binding to promoter-associated non-coding RNA
Chemical Communications
Thermoresponsive Micellar Assembly Constructed from a Hexameric
Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System
Journal of the American
Chemical Society
Schizorhodopsins: A family of rhodopsins from Asgard archaea that
function as light-driven inward H + pumps
Science Advances
Two-State Exchange Dynamics in Membrane-Embedded
Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM
Journal of Molecular Biology
Thermoresponsive structural changes of single poly(N-isopropyl
acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate
Polymer Journal
High-Speed Atomic Force Microscopy Reveals Factors Affecting the
Processivity of Chitinases during Interfacial Enzymatic Hydrolysis of Crystalline Chitin
ACS Catalysis
Rad50 zinc hook functions as a constitutive dimerization module
interchangeable with SMC hinge
Nature Communications
Assembly mechanism of a supramolecular MS-ring complex to
initiate bacterial flagellar biogenesis in vibrio species
Journal of Bacteriology
Structural Dynamics of a Protein Domain Relevant to the Water-
Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy
Journal of Physical Chemistry B
Recent advances in bioimaging with high-speed atomic force
microscopy
Biophysical Reviews
Dynamic behavior of an artificial protein needle contacting a
membrane observed by high-speed atomic force microscopy
Nanoscale
Enhanced enzymatic activity exerted by a packed assembly of a
single type of enzyme
Chemical Science
High Speed AFM and NanoInfrared Spectroscopy Investigation of A
β1–42?Peptide?Variants?and?Their?Interaction?With
POPC/SM/Chol/GM1 Model Membranes
Frontiers in Molecular
Biosciences
The hierarchical assembly of septins revealed by high-speed AFMNature Communications
Millisecond dynamics of an unlabeled amino acid transporterNature Communications
Atg9 is a lipid scramblase that mediates autophagosomal membrane
expansion
Nature Structural & Molecular
Biology
A Simplified Cluster Analysis of Electron Track Structure for
Estimating Complex DNA Damage Yields
International Journal of
Molecular Sciences
Supramolecular tholos-like architecture constituted by archaeal
proteins without functional annotation
Scientific Reports
Studies on the impellers generating force in muscleBiophysical Reviews
Diversity of physical properties of bacterial extracellular membrane
vesicles revealed through atomic force microscopy phase imaging
Nanoscale
High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes
Nano Letters
DNA density-dependent uptake of DNA origami-based two-or three-
dimensional nanostructures by immune cells
Nanoscale
Spatiotemporally tracking of nano-biofilaments inside the nuclear
pore complex core
Biomaterials
High-speed atomic force microscopy directly visualizes
conformational dynamics of the HIV Vif protein in complex with three host proteins
Journal of Biological Chemistry
Self-?and?Cross-Seeding?on?α-Synuclein?Fibril?Growth?Kinetics?and
Structure Observed by High-Speed Atomic Force Microscopy
ACS Nano
Liquidity Is a Critical Determinant for Selective Autophagy of Protein
Condensates
Molecular Cell
Capturing transient antibody conformations with DNA origami
epitopes
Nature Communications
Biophysics in Kanazawa UniversityBiophysical Reviews
Dynamics of oligomer and amyloid fibril formation by yeast prion
Sup35 observed by high-speed atomic force microscopy
Proceedings of the National
Academy of Sciences
Annexin-V stabilizes membrane defects by inducing lipid phase
transition
Nature Communications
Structure and mechanism of bactericidal mammalian perforin-2, an
ancient agent of innate immunity
Science Advances
Atomic Force Microscopy Visualizes Mobility of Photosynthetic
Proteins in Grana Thylakoid Membranes
Biophysical Journal
Construction of a Hexameric Hemoprotein Sheet and Direct
Observation of Dynamic Processes of Its Formation
Chemistry Letters
Single-molecule imaging analysis reveals the mechanism of a high-
catalytic-activity mutant of chitinase A from Serratia marcescens
Journal of Biological Chemistry
Direct observation and analysis of TET-mediated oxidation
processes in a DNA origami nanochip
Nucleic Acids Research
On-membrane dynamic interplay between anti-GM1 IgG antibodies
and complement component C1q
International Journal of
Molecular Sciences
Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening
Function for Cellulose Crystals
Biomacromolecules






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